Department of Pharmaceutical Chemistry and Drug Control, Faculty of Pharmacy, Wadi International University, Homs, Syria
Abstract
An innovative, uncomplicated, accurate, and precise reverse-phase high-performance liquid chromatography method has been established for the determination of Vildagliptin in its bulk form and in pharmaceuticals. The separation was realized on a mobile phase of acetonitrile: ammonium dihydrogen phosphate (10: 90, v/v). The detection was achieved at 200 nm and the flow rate was 1.5 mL.min-1. Under the optimized conditions, short retention times (~3.5 min) were attained. Such a value permits for the rapid determination of multiple samples within a minimal timeframe, enhancing the efficiency of the analytical process. In such a way, the assay should be economical and can be used feasibly in routine pharmaceutical analysis. Furthermore, the limit of detection and limit of quantification values were 1.158 μg.mL-1 and 3.509 μg.mL-1 respectively, indicating a sensitive determination. Finally, the method has proven to be linear in the range of 40-160 μg.mL-1, demonstrating its reliability and robustness for consistent analytical performance.
Darwish, M. (2024). Efficient RP-HPLC Method for the Detection and Quantification of Vildagliptin in Bulk and Pharmaceutical Formulations. Eurasian Journal of Chemical, Medicinal and Petroleum Research, 3(3), 1064–1074.
MLA
Darwish, M. "Efficient RP-HPLC Method for the Detection and Quantification of Vildagliptin in Bulk and Pharmaceutical Formulations", Eurasian Journal of Chemical, Medicinal and Petroleum Research, 3, 3, 2024, 1064–1074.
HARVARD
Darwish, M. (2024). 'Efficient RP-HPLC Method for the Detection and Quantification of Vildagliptin in Bulk and Pharmaceutical Formulations', Eurasian Journal of Chemical, Medicinal and Petroleum Research, 3(3), pp. 1064–1074.
CHICAGO
Darwish, M., "Efficient RP-HPLC Method for the Detection and Quantification of Vildagliptin in Bulk and Pharmaceutical Formulations." Eurasian Journal of Chemical, Medicinal and Petroleum Research, 3 3 (2024): 1064–1074,
VANCOUVER
Darwish M. Efficient RP-HPLC Method for the Detection and Quantification of Vildagliptin in Bulk and Pharmaceutical Formulations. Eurasian J. Chem. Med. Pet. Res. 2024;3(3):1064–1074.
